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Ancient bacteria show evidence of DNA repair

Författare:
  • Sarah Stewart Johnson
  • Martin B. Hebsgaard
  • Torben Christensen
  • Mikhail Mastepanov
  • Rasmus Nielsen
  • Kasper Munch
  • Tina Brand
  • M. Thomas
  • P. Gilbert
  • Maria T. Zuber
  • Michael Bunce
  • Regin Ronn
  • David Gilichinsky
  • Duane Froese
  • Eske Willerslev
Publiceringsår: 2007
Språk: Engelska
Sidor: 14401-14405
Publikation/Tidskrift/Serie: Proceedings of the National Academy of Sciences of the United States of America
Volym: 104
Nummer: 36
Dokumenttyp: Artikel
Förlag: National Academy of Sciences

Sammanfattning

Recent claims of cultivable ancient bacteria within sealed environments highlight our limited understanding of the mechanisms behind long-term cell survival. It remains unclear how dormancy, a favored explanation for extended cellular persistence, can cope with spontaneous genomic decay over geological timescales. There has been no direct evidence in ancient microbes for the most likely mechanism, active DNA repair, or for the metabolic activity necessary to sustain it. In this paper, we couple PCR and enzymatic treatment of DNA with direct respiration measurements to investigate long-term survival of bacteria sealed in frozen conditions for up to one million years. Our results show evidence of bacterial survival in samples up to half a million years in age, making this the oldest independently authenticated DNA to date obtained from viable cells. Additionally, we find strong evidence that this long-term survival is closely tied to cellular metabolic activity and DNA repair that over time proves to be superior to dormancy as a mechanism in sustaining bacteria viability.

Disputation

Nyckelord

  • Biology and Life Sciences
  • DNA damage
  • long-term microbial survival
  • metabolic activity

Övrigt

Published
Yes
  • ISSN: 0027-8424

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