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Development of reagents for differential protein quantitation by subtractive parent (precursor) ion scanning

Författare:
  • Åsa Wåhlander (PhD)
  • Giorgio Arrigoni
  • Kristofer Wårell
  • Fredrik Levander
  • Ronnie Palmgren
  • Jean-Luc Maloisel
  • Philippe Busson
  • Peter James
Publiceringsår: 2007
Språk: Engelska
Sidor: 1101-1113
Publikation/Tidskrift/Serie: Journal of Proteome Research
Volym: 6
Nummer: 3
Dokumenttyp: Artikel
Förlag: American Chemical Society

Sammanfattning

We present a generic approach for quantitative differential proteomics that reduces data complexity in proteome analysis by automated selection of peptides for MS/MS analysis according to their isotope-labeling ratio. Isotopic reagents were developed that give products which fragment easily to generate a unique pair of signature ions. Using the ion-pair ratio, we show that it is possible to select only BSA peptides (with a 3:1 light heavy isotope ratio) for MS/MS when spiked in a whole yeast extract using Parent (precursor) Ion Quantitation Scanning (PIQS) for MS/MS.

Disputation

Nyckelord

  • Technology and Engineering
  • precursor-ion
  • reagents
  • mass spectrometry
  • isotope labeling
  • HPLC
  • parent-ion

Övriga

Published
Yes
  • ISSN: 1535-3893

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