Development of reagents for differential protein quantitation by subtractive parent (precursor) ion scanning

Summary, in English

We present a generic approach for quantitative differential proteomics that reduces data complexity in proteome analysis by automated selection of peptides for MS/MS analysis according to their isotope-labeling ratio. Isotopic reagents were developed that give products which fragment easily to generate a unique pair of signature ions. Using the ion-pair ratio, we show that it is possible to select only BSA peptides (with a 3:1 light heavy isotope ratio) for MS/MS when spiked in a whole yeast extract using Parent (precursor) Ion Quantitation Scanning (PIQS) for MS/MS.

• Författare: Åsa Wåhlander; Giorgio Arrigoni; Kristofer Wårell; Fredrik Levander; Ronnie Palmgren; Jean-Luc Maloisel; Philippe Busson; Peter James
• Avdelning/ar: Institutionen för immunteknologi
• Publiceringsår: 2007
• Språk: Engelska
• Sidor: 1101-1113
• Publikation/Tidskrift/Serie: Journal of Proteome Research
• Volym: 6
• Issue: 3
• Länkar: http://dx.doi.org/10.1021/pr0604312
• Dokumenttyp: Artikel i tidskrift
• Förlag: The American Chemical Society (ACS)
• Ämne: Immunology in the medical area
• Nyckelord: precursor-ion; reagents; mass spectrometry; isotope labeling; HPLC; parent-ion
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1535-3893