Transcription factor profiling in individual hematopoietic progenitors by digital RT-PCR
Författare
Summary, in English
We report here a systematic, quantitative population analysis of transcription factor expression within developmental progenitors, made possible by a microfluidic chip-based "digital RT-PCR" assay that can count template molecules in cDNA samples prepared from single cells. In a survey encompassing five classes of early hematopoietic precursor, we found markedly heterogeneous expression of the transcription factor PU.1 in hematopoietic stem cells and divergent patterns of PU.1 expression within flk2(-) and flk2(+) common myeloid progenitors. The survey also revealed significant differences in the level of the housekeeping transcript GAPDH across the surveyed populations, which demonstrates caveats of normalizing expression data to endogenous controls and underscores the need to put gene measurement on an absolute, copy-per-cell basis.
Avdelning/ar
Publiceringsår
2006
Språk
Engelska
Sidor
17807-17812
Publikation/Tidskrift/Serie
Proceedings of the National Academy of Sciences
Volym
103
Issue
47
Dokumenttyp
Artikel i tidskrift
Förlag
National Academy of Sciences
Ämne
- Cell and Molecular Biology
Nyckelord
- PU.1
- microfluidics
- gene profiling
- hematopoiesis
- stem cells
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1091-6490