Meny

Du är här

Calpain activation and apoptosis in motor neurons of cultured adult mouse spinal cord

Författare:
  • Hamid Reza Momeni
  • Seifollah Azadi
  • Martin Kanje
Publiceringsår: 2007
Språk: Engelska
Sidor: 105-110
Publikation/Tidskrift/Serie: Functional Neurology
Volym: 22
Nummer: 2
Dokumenttyp: Artikel
Förlag: CIC Edizioni Internazionali

Sammanfattning

Calpain, a Ca2+-dependent cysteine protease, has been implicated in neuronal apoptosis following spinal cord injury. In this study, activation of calpain was investigated in motor neurons of adult spinal cord slices from the mouse, using a cell-permeable fluorogenic calpain substrate and Western blotting. Calpain was rapidly activated in the motor neurons of excised spinal cord slices and calpain activity was observed both in the cytoplasm and the nuclei. In these neurons, nuclear and chromatin condensation were pronounced. Both calpain inhibitor VI and EGTA (ethyleneglycol-bis(beta-aminoethyl ether) N',N',N',N'-tetraacetic acid) inhibited calpain activation and subsequent appearance of apoptotic nuclei. In contrast, the general caspase inhibitor Z-VAD.fmk had no effect. Calpain activation was also observed in the slices by Western blotting using an antibody to 150-kD calpain-cleaved a-fodrin fragment. These results show that calpain is rapidly activated in injured motor neurons and imply that this activation could be responsible for execution of caspase-independent apoptosis in injured adult motor neurons.

Disputation

Nyckelord

  • Biology and Life Sciences
  • organ culture
  • motor neurons
  • caspase
  • apoptosis
  • calpain
  • spinal cord

Övriga

Published
Yes
  • ISSN: 0393-5264

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen