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Novel anti-apoptotic effect of Bcl-2: Prevention of polyamine depletion-induced cell death

Författare:
  • Martina Holst (PhD)
  • Veronica Johansson (PhD student)
  • Kersti Alm (Dr.)
  • Stina Oredsson
Publiceringsår: 2008
Språk: Engelska
Sidor: 66-74
Publikation/Tidskrift/Serie: Cell Biology International
Volym: 32
Dokumenttyp: Artikel
Förlag: Elsevier

Sammanfattning

The spermine analogue N(1),N(11)-diethylnorspermine (DENSPM) efficiently depletes the polyamine pools in the breast cancer cell line L56Br-C1 and induces apoptotic cell death via the mitochondrial pathway. In this study, we have over-expressed the anti-apoptotic protein Bcl-2 in L56Br-C1 cells and investigated the effect of DENSPM treatment. DENSPM-induced cell death was significantly reduced in Bcl-2 over-expressing cells. Bcl-2 over-expression reduced DENSPM-induced release of the pro-apoptotic proteins AIF, cytochrome c, and Smac/DIABLO from the mitochondria. Bcl-2 over-expression reduced the DENSPM-induced activation of caspase-3. Bcl-2 over-expression also prevented DENSPM-induced Bax cleavage and reduction of Bcl-X(L) and survivin levels. The DENSPM-induced activation of the polyamine catabolic enzyme spermidine/spermine N(1)-acetyltransferase was reduced by Bcl-2 over-expression, partly preventing polyamine depletion. Thus, Bcl-2 over-expression prevented a number of DENSPM-induced apoptotic effects

Disputation

Nyckelord

  • Biology and Life Sciences
  • Apoptosis
  • Spermine analogue
  • Cytochrome c
  • Human breast cancer
  • Bcl-2

Övrigt

Published
Yes
  • ISSN: 1095-8355
  • ISSN: 1065-6995

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