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Tyrosine hydroxylase expression is unstable in a human immortalized mesencephalic cell line - Studies in vitro and after intracerebral grafting in vivo

Författare

Summary, in English

We have studied the stability of the dopaminergic phenotype in a conditionally immortalized human mesencephalic cell line, NIESC2.10. Even though MESC2.10 cells exhibit features of dopaminergic neurons in vitro, none of the cells expressed tyrosine hydroxylase (TH) after transplantation into a rat model of Parkinson's disease. We examined whether this is caused by cell death or loss of transmitter phenotype. Cells were cultured in differentiation medium, then harvested and replated into the same medium where they continued to express TH, whereas replated cells fed medium lacking differentiation factors (dibutyryl cAMP and glial cell line-derived neurotrophic factor) did not. Interestingly, cultures grown in the absence of differentiation factors could regain TH expression once exposed to differentiation medium. Our data suggest that TH expression in vitro is inducible in neurons derived from the NIESC2.10 cell line and that the dopaminergic phenotype of these cells in vivo might be unstable. (c) 2006 Elsevier Inc. All rights reserved.

Publiceringsår

2007

Språk

Engelska

Sidor

390-399

Publikation/Tidskrift/Serie

Molecular and Cellular Neuroscience

Volym

34

Issue

3

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Neurosciences

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1044-7431