Novel antibody specificities targeting glycoprotein B of cytomegalovirus identified by molecular library technology
Författare
Summary, in English
Antibodies provide some protection against cytomegalovirus-mediated disease. All aspects of antibody recognition of important viral antigens are however not fully appreciated. Glycoprotein B (gB), a key protein in the viral membrane, participates in viral infection and it is a component of prototype vaccines. By using combinatorial antibody library and selection technology, novel antibody specificities targeting gB have now been isolated. We define a monoclonal antibody fragment able to recognize site I of antigenic domain (AD) 2, a poorly immunogenic epitope targeted by potent virus-neutralizing antibodies, in a way that is different from the binding of antibodies induced by infection but similar to those induced by vaccination. We also describe the existence of a novel epitope overlapping site I of AD-2 and AD-1, the immunodominant epitope of gB. These specificities, derived by molecular engineering, will be useful for the future assessment of humoral immune responses against this opportunistic viral infection.
Avdelning/ar
Publiceringsår
2009
Språk
Engelska
Sidor
429-436
Publikation/Tidskrift/Serie
New Biotechnology
Volym
25
Issue
6
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Immunology in the medical area
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1876-4347