Evaluation of quantitative thin layer chromatography using staining reagents

Summary, in English

Thin layer chromatography (TLC) using staining reagents is a superior method for analyzing organic compounds without chromophores. It is fast, versatile and sometimes the only viable method. We have investigated quantitative TLC using staining reagents, in combination with modem image analysis software. Our results show that it is possible to get reliable measurements, suitable for high-throughput screening or physical organic investigations. The range of detection and the errors for the different parts of the process are illustrated. We show that the errors are largely due to the staining process and can be diminished by measuring ratios of compounds. (c) 2007 Elsevier B.V. All rights reserved.

• Författare: Richard Johnsson; Gustav Traff; Martin Sundén; Ulf Ellervik
• Avdelning/ar: Centrum för analys och syntes
• Publiceringsår: 2007
• Språk: Engelska
• Sidor: 298-305
• Publikation/Tidskrift/Serie: Journal of Chromatography A
• Volym: 1164
• Issue: 1-2
• Länkar: http://dx.doi.org/10.1016/j.chroma.2007.07.029
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Organic Chemistry
• Nyckelord: quantification; TLC; image analysis software; staining reagents
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 0021-9673