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Versatile microchip utilising ultrasonic standing waves

Publiceringsår: 2005
Språk: Engelska
Sidor: 123-124
Publikation/Tidskrift/Serie: IFMBE Proceedings 2005
Dokumenttyp: Konferensbidrag


This paper presents the concept and initial work on a
microfluidic platform for bead-based analysis of
biological sample. The core technology in this project
is ultrasonic manipulation and trapping of particle in
array configurations by means of acoustic forces. The
platform is ultimately aimed for parallel multistep
bioassays performed on biochemically activated
microbeads (or particles) using submicrolitre sample
volumes. A first prototype with three individually
controlled particle trapping sites has been developed
and evaluated. Standing ultrasonic waves were
generated across a microfluidic channel by integrated
PZT ultrasonic microtransducers. Particles in a fluid
passing a transducer were drawn to pressure minima
in the acoustic field, thereby being trapped and
confined laterally over the transducer. It is
anticipated that acoustic trapping using integrated
transducers can be exploited in miniaturised total
chemical analysis systems (µTAS), where e.g.
microbeads with immobilised antibodies can be
trapped in arrays and subjected to minute amounts of
sample followed by a reaction, detected using
fluorescence. Preliminary results indicate that the
platform is capable of handling live cells as well as
microbeads. A first model bioassay with detection of
fluorescein marked avidin binding to trapped biotin
beads has been evaluated.



  • Medical Engineering


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