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Integrin Based Isolation Enables Purification of Murine Lineage Committed Cardiomyocytes

Författare

  • Laura Tarnawski
  • Xiaojie Xian
  • Gustavo Monnerat
  • Iain C. Macaulay
  • Daniela Malan
  • Andrew Borgman
  • Sean M. Wu
  • Bernd K. Fleischmann
  • Stefan Jovinge

Summary, in English

In contrast to mature cardiomyocytes which have limited regenerative capacity, pluripotent stem cells represent a promising source for the generation of new cardiomyocytes. The tendency of pluripotent stem cells to form teratomas and the heterogeneity fromvarious differentiation stages and cardiomyocyte cell sub-types, however, are major obstacles to overcome before this type of therapy could be applied in a clinical setting. Thus, the identification of extracellularmarkers for specific cardiomyocyte progenitors and mature subpopulations is of particular importance. The delineation of cardiomyocyte surfacemarker patterns not only serves as a means to derive homogeneous cell populations by FACS, but is also an essential tool to understand cardiac development. By using single-cell expression profiling in early mouse embryonic hearts, we found that a combination of integrin alpha-1, alpha-5, alpha-6 and N-cadherin enables isolation of lineage committed murine cardiomyocytes. Additionally, we were able to separate trabecular cardiomyocytes from solid ventricular myocardium and atrial murine cells. These cells exhibit expected subtype specific phenotype confirmed by electrophysiological analysis. We show that integrin expression can be used for the isolation of living, functional and lineage-specific murine cardiomyocytes.

Publiceringsår

2015

Språk

Engelska

Publikation/Tidskrift/Serie

PLoS ONE

Volym

10

Issue

8

Dokumenttyp

Artikel i tidskrift

Förlag

Public Library of Science (PLoS)

Ämne

  • Cardiac and Cardiovascular Systems

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1932-6203