Phylogenetic analysis and substrate specificity of GH2 β-mannosidases from Aspergillus species.
Författare
Summary, in English
Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised β-mannosidases from other organisms, clusters putative Aspergillus β-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched β-mannosides like mannobiose. A 3D-model of MndB provides further insight.
Avdelning/ar
Publiceringsår
2013
Språk
Engelska
Sidor
3444-3449
Publikation/Tidskrift/Serie
FEBS Letters
Volym
587
Issue
21
Dokumenttyp
Artikel i tidskrift
Förlag
Wiley-Blackwell
Ämne
- Biological Sciences
Status
Published
Projekt
- Galactomannan degradation by fungi and gut bacteria
ISBN/ISSN/Övrigt
- ISSN: 1873-3468