Acoustophoretic removal of proteins from blood components.

Summary, in English

This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97 %, compared to just above 90 % of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications.

• Författare: Maria Tenje; Maria Lundgren; Ann-Margret Swärd-Nilsson; Jens Kjeldsen-Kragh; Lena Lyxe; Andreas Lenshof
• Avdelning/ar: Avdelningen för mikrobiologi, immunologi och glykobiologi - MIG; Avdelningen för hematologi och transfusionsmedicin; Avdelningen för Biomedicinsk teknik
• Publiceringsår: 2015
• Språk: Engelska
• Sidor: 95-95
• Publikation/Tidskrift/Serie: Biomedical Microdevices
• Volym: 17
• Issue: 5
• Länkar: http://www.ncbi.nlm.nih.gov/pubmed/26315975?dopt=Abstract; http://dx.doi.org/10.1007/s10544-015-0003-5
• Dokumenttyp: Artikel i tidskrift
• Förlag: Springer
• Ämne: Biomedical Laboratory Science/Technology
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1572-8781