Surface nanostructures for fluorescence probing of supported lipid bilayers on reflective substrates.
Författare
Summary, in English
The fluorescence interference contrast (FLIC) effect prevents the use of fluorescence techniques to probe the continuity and fluidity of supported lipid bilayers on reflective materials due to a lack of detectable fluorescence. Here we show that adding nanostructures onto reflective surfaces to locally confer a certain distance between the deposited fluorophores and the reflecting surface enables fluorescence detection on the nanostuctures. The nanostructures consist of either deposited nanoparticles or epitaxial nanowires directly grown on the substrate and are designed such that they can support a lipid bilayer. This simple method increases the fluorescence signal sufficiently to enable bilayer fluorescence detection and to observe the recovery of fluorescence after photobleaching in order to assess lipid bilayer formation on any reflective surface.
Avdelning/ar
Publiceringsår
2015
Språk
Engelska
Sidor
18020-18024
Publikation/Tidskrift/Serie
Nanoscale
Volym
7
Issue
43
Fulltext
Dokumenttyp
Artikel i tidskrift
Förlag
Royal Society of Chemistry
Ämne
- Biophysics
Status
Published
Forskningsgrupp
- Neuronano Research Center (NRC)
ISBN/ISSN/Övrigt
- ISSN: 2040-3372