Proposed lipocalin fold for apolipoprotein M based on bioinformatics and site-directed mutagenesis
Författare
Summary, in English
Apolipoprotein RI (apoM) is a novel apolipoprotein that is predominantly present in high-density lipoprotein, Sensitive sequence starches, threading and comparative model building experiments revealed apoM to be structurally related to the lipocalin protein family. In a 3D model, characterized by an eight-stranded anti-parallel beta -barrel, a segment including Asn135 could adopt a closed or open conformation. Using site-directed mutagenesis, we demonstrated Asn135 in wild-type apoM to be glycosylated, suggesting that the segment is solvent exposed, ApoM displays two strong acidic patches of potential functional importance, one around the N-terminus and the other next to the opening of the beta -barrel.
Avdelning/ar
Publiceringsår
2001
Språk
Engelska
Sidor
127-132
Publikation/Tidskrift/Serie
FEBS Letters
Volym
499
Issue
1-2
Dokumenttyp
Artikel i tidskrift
Förlag
Wiley-Blackwell
Ämne
- Biological Sciences
Nyckelord
- Lipocalin
- Apolipoprotein M
- Comparative modeling
- Site-directed mutagenesis
Status
Published
Forskningsgrupp
- Clinical Chemistry, Malmö
ISBN/ISSN/Övrigt
- ISSN: 1873-3468