From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behaviour.
Författare
Summary, in English
The field of vertical nanowire array-based applications in cell biology is growing rapidly and an increasing number of applications are being explored. These applications almost invariably rely on the physical properties of the nanowire arrays, creating a need for a better understanding of how their physical properties affect cell behaviour. Here, we investigate the effects of nanowire density on cell migration, division and morphology for murine fibroblasts. Our results show that few nanowires are sufficient to immobilize cells, while a high nanowire spatial density enables a "bed-of-nails" regime, where cells reside on top of the nanowires and are fully motile. The presence of nanowires decreases the cell proliferation rate, even in the "bed-of-nails" regime. We show that the cell morphology strongly depends on the nanowire density. Cells cultured on low (0.1 μm(-2)) and medium (1 μm(-2)) density substrates exhibit an increased number of multi-nucleated cells and micronuclei. These were not observed in cells cultured on high nanowire density substrates (4 μm(-2)). The results offer important guidelines to minimize cell-function perturbations on nanowire arrays. Moreover, these findings offer the possibility to tune cell proliferation and migration independently by adjusting the nanowire density, which may have applications in drug testing.
Avdelning/ar
- Fasta tillståndets fysik
- NanoLund: Centre for Nanoscience
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- Funktionell zoologi
- Neuronano Research Center (NRC)
Publiceringsår
2015
Språk
Engelska
Publikation/Tidskrift/Serie
Scientific Reports
Volym
5
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Nature Publishing Group
Ämne
- Medical Biotechnology
Status
Published
Forskningsgrupp
- Neuronano Research Center (NRC)
ISBN/ISSN/Övrigt
- ISSN: 2045-2322