MALDI-target integrated platform for affinity-captured protein digestion.
Författare
Summary, in English
To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40fmol to 1pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented.
Avdelning/ar
Publiceringsår
2014
Språk
Engelska
Sidor
1-8
Publikation/Tidskrift/Serie
Analytica Chimica Acta
Volym
807
Issue
Jan 7
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Analytical Chemistry
Status
Published
Forskningsgrupp
- Clinical Chemistry, Malmö
ISBN/ISSN/Övrigt
- ISSN: 1873-4324