A sex pheromone receptor in the Hessian fly Mayetiola destructor (Diptera, Cecidomyiidae)
Författare
Summary, in English
The Hessian fly, Mayetiola destructor Say (Diptera,
Cecidomyiidae), is a pest of wheat and belongs to a group of
gall-inducing herbivores. This species has a unique life history and
several ecological features that differentiate it from other Diptera
such as Drosophila melanogaster and blood-feeding mosquitoes.
These features include a short, non-feeding adult life stage (1–2 days)
and the use of a long-range sex pheromone produced and released by adult
females. Sex pheromones are detected by members of the odorant receptor
(OR) family within the Lepidoptera, but no receptors for similar
long-range sex pheromones have been characterized from the Diptera.
Previously, 122 OR genes have been annotated from the Hessian fly
genome, with many of them showing sex-biased expression in the antennae.
Here we have expressed, in HEK293 cells, five MdesORs that display
male-biased expression in antennae, and we have identified MdesOR115 as a
Hessian fly sex pheromone receptor. MdesOR115 responds primarily to the
sex pheromone component (2S,8E,10E)-8,10-tridecadien-2-yl acetate, and secondarily to the corresponding Z,E-isomer.
Certain sensory neuron membrane proteins (i.e., SNMP1) are important
for responses of pheromone receptors in flies and moths. The Hessian fly
genome is unusual in that it encodes six SNMP1 paralogs, of which five
are expressed in antennae. We co-expressed each of the five antennal
SNMP1 paralogs together with each of the five candidate sex pheromone
receptors from the Hessian fly and found that they do not influence the
response of MdesOR115, nor do they confer responsiveness in any of the
non-responsive ORs to any of the sex pheromone components identified to
date in the Hessian fly. Using Western blots, we detected protein
expression of MdesOrco, all MdesSNMPs, and all MdesORs except for
MdesOR113, potentially explaining the lack of response from this OR. In
conclusion, we report the first functional characterization of an OR
from the Cecidomyiidae, extending the role of ORs as long-range sex
pheromone detectors from the Lepidoptera into the Diptera.
Cecidomyiidae), is a pest of wheat and belongs to a group of
gall-inducing herbivores. This species has a unique life history and
several ecological features that differentiate it from other Diptera
such as Drosophila melanogaster and blood-feeding mosquitoes.
These features include a short, non-feeding adult life stage (1–2 days)
and the use of a long-range sex pheromone produced and released by adult
females. Sex pheromones are detected by members of the odorant receptor
(OR) family within the Lepidoptera, but no receptors for similar
long-range sex pheromones have been characterized from the Diptera.
Previously, 122 OR genes have been annotated from the Hessian fly
genome, with many of them showing sex-biased expression in the antennae.
Here we have expressed, in HEK293 cells, five MdesORs that display
male-biased expression in antennae, and we have identified MdesOR115 as a
Hessian fly sex pheromone receptor. MdesOR115 responds primarily to the
sex pheromone component (2S,8E,10E)-8,10-tridecadien-2-yl acetate, and secondarily to the corresponding Z,E-isomer.
Certain sensory neuron membrane proteins (i.e., SNMP1) are important
for responses of pheromone receptors in flies and moths. The Hessian fly
genome is unusual in that it encodes six SNMP1 paralogs, of which five
are expressed in antennae. We co-expressed each of the five antennal
SNMP1 paralogs together with each of the five candidate sex pheromone
receptors from the Hessian fly and found that they do not influence the
response of MdesOR115, nor do they confer responsiveness in any of the
non-responsive ORs to any of the sex pheromone components identified to
date in the Hessian fly. Using Western blots, we detected protein
expression of MdesOrco, all MdesSNMPs, and all MdesORs except for
MdesOR113, potentially explaining the lack of response from this OR. In
conclusion, we report the first functional characterization of an OR
from the Cecidomyiidae, extending the role of ORs as long-range sex
pheromone detectors from the Lepidoptera into the Diptera.
Avdelning/ar
Publiceringsår
2016-09-07
Språk
Engelska
Publikation/Tidskrift/Serie
Frontiers in Cellular Neuroscience
Volym
10
Issue
SEP2016
Fulltext
Dokumenttyp
Artikel i tidskrift
Förlag
Frontiers Media S. A.
Ämne
- Biochemistry and Molecular Biology
- Cell Biology
Nyckelord
- deorphanization
- functional characterization
- heterologous expression
- HEK293 cells
- odorant receptor
- pheromone receptor
- sensory neuron membrane protein
Status
Published
Projekt
- Sex pheromone biosynthesis and odorant receptors in gall midges
Forskningsgrupp
- Pheromone Group
ISBN/ISSN/Övrigt
- ISSN: 1662-5102