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Comparison of different extraction techniques for isolation of antioxidants from sweet grass (Hierochloe odorata)

Författare

Summary, in English

Strong antioxidants, 5,8-dihydroxycoumarin and 5-hydroxy-8-O-beta-D-glucopyranosyl-benzopyranone were extracted from sweet grass (Hierochloe odorata L.) by using Soxhlet (SE), microwave-assisted (MAE) and supercritical fluid carbon dioxide (SFE) extractions at different extraction schemes and parameters. One-step MAE gave the most concentrated extract with 8.15% of 5,8-dihydroxycoumarin (extract yield 0.42%). Comparable results were obtained by one-step SFE and Soxhlet extraction. A two-step extraction was applied in order to increase the concentration of active compounds. Taking into account antioxidant recovery and concentration as well the extraction time and environmental aspects, two-step SFE was considered as a preferable method. The obtained crude extract contained 20.31% of 5,8-dihydroxycoumarin and 2.18% of 5-hydroxy-8-O-beta-D-glucopyranosyl-benzopyranone. The extraction yields were 0.46 and 0.06%, respectively. First-step of SFE extraction was performed from dried herb at 35 MPa and 40degreesC with 20% of ethanol as a modifier; the second extraction step was performed from the crude extract at 25 MPa and 40degreesC.

Publiceringsår

2005

Språk

Engelska

Sidor

223-233

Publikation/Tidskrift/Serie

Journal of Supercritical Fluids

Volym

33

Issue

3

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Analytical Chemistry
  • Engineering and Technology

Nyckelord

  • sweet grass
  • extraction (MAE)
  • microwave-assisted
  • soxhlet
  • supercritical fluid extraction (SFE)
  • Hierochloe odorata
  • antioxidants

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0896-8446