Derivation, characterization, and differentiation of human embryonic stem cells
Författare
Summary, in English
The derivation of human embryonic stem (hES) cells establishes a new avenue to approach many issues in human biology and medicine for the first time. To meet the increased demand for characterized hES cell lines, we present the derivation and characterization of six hES cell lines. In addition to the previously described immunosurgery procedure, we were able to propagate the inner cell mass and establish hES cell lines from pronasetreated and hatched blastocysts. The cell lines were extensively characterized by expression analysis of markers gated in an undiffer characteristic for undifferentiated and differentiated hES cells, karyotyping, telomerase activity measurement, and pluripotency assays in vitro and in vivo. Whereas three of the cell lines expressed all the characteristics of undifferentiated pluripotent hES cells, one cell line carried a chromosome 13 trisomy while maintaining an undifferentiated pluripotent state, and two cell lines, one of which carried a triploid karyotype, exhibited limited pluripotency in vivo. Furthermore, we clonally derived one cell line, which could be propaentiated pluripotent state.
Publiceringsår
2004
Språk
Engelska
Sidor
367-376
Publikation/Tidskrift/Serie
Stem Cells
Volym
22
Issue
3
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
AlphaMed Press
Ämne
- Cell and Molecular Biology
Nyckelord
- differentiation
- pluripotency
- human ES cell
- human blastocyst
- ICM
- subcloning
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1549-4918