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Derivation, characterization, and differentiation of human embryonic stem cells

Författare

  • N Heins
  • MCO Englund
  • C Sjoblom
  • U Dahl
  • A Tonning
  • C Bergh
  • A Lindahl
  • C Hanson
  • Henrik Semb

Summary, in English

The derivation of human embryonic stem (hES) cells establishes a new avenue to approach many issues in human biology and medicine for the first time. To meet the increased demand for characterized hES cell lines, we present the derivation and characterization of six hES cell lines. In addition to the previously described immunosurgery procedure, we were able to propagate the inner cell mass and establish hES cell lines from pronasetreated and hatched blastocysts. The cell lines were extensively characterized by expression analysis of markers gated in an undiffer characteristic for undifferentiated and differentiated hES cells, karyotyping, telomerase activity measurement, and pluripotency assays in vitro and in vivo. Whereas three of the cell lines expressed all the characteristics of undifferentiated pluripotent hES cells, one cell line carried a chromosome 13 trisomy while maintaining an undifferentiated pluripotent state, and two cell lines, one of which carried a triploid karyotype, exhibited limited pluripotency in vivo. Furthermore, we clonally derived one cell line, which could be propaentiated pluripotent state.

Publiceringsår

2004

Språk

Engelska

Sidor

367-376

Publikation/Tidskrift/Serie

Stem Cells

Volym

22

Issue

3

Dokumenttyp

Artikel i tidskrift

Förlag

AlphaMed Press

Ämne

  • Cell and Molecular Biology

Nyckelord

  • differentiation
  • pluripotency
  • human ES cell
  • human blastocyst
  • ICM
  • subcloning

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1549-4918