A new nested polymerase chain reaction method very efficient in detecting Plasmodium and Haemoproteus infections from avian blood
Författare
Summary, in English
Recently, several polymerase chain reaction (PCR)-based methods for detection and genetic identification of haemosporidian parasites in avian blood have been developed. Most of these have considerably higher sensitivity compared with traditional microscope-based examinations of blood smears. These new methods have already had a strong impact on several aspects of research on avian blood parasites. In this study. We present a new nested PCR approach, building on a previously published PCR method, which has significantly improved performance. We compare the new method with some existing assays and show, by sequence-based data, that the higher detection rate is mainly due to superior detection of Plasmodium spp. infections, which often are of low intensity and, therefore, hard to detect with other methods.
Publiceringsår
2004
Språk
Engelska
Sidor
191-194
Publikation/Tidskrift/Serie
The Journal of Parasitology
Volym
90
Issue
1
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
American Society of Parasitologists
Ämne
- Biological Sciences
Status
Published
Projekt
- Malaria in birds
Forskningsgrupp
- Molecular Ecology and Evolution Lab
ISBN/ISSN/Övrigt
- ISSN: 0022-3395