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A new nested polymerase chain reaction method very efficient in detecting Plasmodium and Haemoproteus infections from avian blood

In English

Författare

Summary, in English

Recently, several polymerase chain reaction (PCR)-based methods for detection and genetic identification of haemosporidian parasites in avian blood have been developed. Most of these have considerably higher sensitivity compared with traditional microscope-based examinations of blood smears. These new methods have already had a strong impact on several aspects of research on avian blood parasites. In this study. We present a new nested PCR approach, building on a previously published PCR method, which has significantly improved performance. We compare the new method with some existing assays and show, by sequence-based data, that the higher detection rate is mainly due to superior detection of Plasmodium spp. infections, which often are of low intensity and, therefore, hard to detect with other methods.

Publiceringsår

2004

Språk

Engelska

Sidor

191-194

Publikation/Tidskrift/Serie

The Journal of Parasitology

Volym

90

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

American Society of Parasitologists

Ämne

  • Biological Sciences

Status

Published

Projekt

  • Malaria in birds

Forskningsgrupp

  • Molecular Ecology and Evolution Lab

ISBN/ISSN/Övrigt

  • ISSN: 0022-3395