The miR-17-92 MicroRNA Cluster Regulates Multiple Components of the TGF-beta Pathway in Neuroblastoma
Författare
Summary, in English
The miR-17-92 microRNA cluster is often activated in cancer cells, but the identity of its targets remains elusive. Using SILAC and quantitative mass spectrometry, we examined the effects of activation of the miR-17-92 cluster on global protein expression in neuroblastoma (NB) cells. Our results reveal cooperation between individual miR-17-92 miRNAs and implicate miR-17-92 in multiple hallmarks of cancer, including proliferation and cell adhesion. Most importantly, we show that miR-17-92 is a potent inhibitor of TGF-beta signaling. By functioning both upstream and downstream of pSMAD2, miR-17-92 activation triggers downregulation of multiple key effectors along the TGF-beta signaling cascade as well as direct inhibition of TGF-beta-responsive genes.
Avdelning/ar
Publiceringsår
2010
Språk
Engelska
Sidor
762-773
Publikation/Tidskrift/Serie
Molecular Cell
Volym
40
Issue
5
Dokumenttyp
Artikel i tidskrift
Förlag
Cell Press
Ämne
- Cancer and Oncology
Status
Published
Forskningsgrupp
- Kidney cancer research group
ISBN/ISSN/Övrigt
- ISSN: 1097-4164