Ubiquinone-1 induces external deamino-NADH oxidation in potato tuber mitochondria
Författare
Summary, in English
The addition of ubiquinone-1 (UQ-1) induced Ca2+-independent oxidation of deamino-NADH and NADH by intact potato (Solanum tuberosum L. cv Bintje) tuber mitochondria. The induced oxidation was coupled to the generation of a membrane potential. Measurements of NAD+-malate dehydrogenase activity indicated that the permeability of the inner mitochondrial membrane to NADH and deamino-NADH was not altered by the addition of UQ-1. We conclude that UQ-1-induced external deamino-NADH oxidation is due to a change in specificity of the external rotenone-insensitive NADH dehydrogenase. The addition of UQ-1 also induced rotenone-insensitive oxidation of deamino-NADH by inside-out submitochondrial particles, but whether this was due to a change in the specificity of the internal rotenone-insensitive NAD(P)H dehydrogenase or to a bypass in complex I could not be determined.
Avdelning/ar
Publiceringsår
1996-09
Språk
Engelska
Sidor
75-78
Publikation/Tidskrift/Serie
Plant Physiology
Volym
112
Issue
1
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
American Society of Plant Biologists
Ämne
- Biochemistry and Molecular Biology
Status
Published
Forskningsgrupp
- Plant Biology
ISBN/ISSN/Övrigt
- ISSN: 0032-0889