Growth kinetics and Pho84 phosphate transporter activity of Saccharomyces cerevisiae under phosphate-limited conditions
Författare
Summary, in English
The effect of phosphate (P ( i )) concentration on the growth behavior of Saccharomyces cerevisiae strain CEN.PK113-5D in phosphate-limited batch and chemostat cultures was studied. The range of dilution rates used in the present study was 0.08-0.45 h(-1). The batch growth of yeast cells followed Monod relationship, but growth of the cells in phosphate-limited chemostat showed change in growth kinetics with increasing dilution rates. The difference in growth kinetics of the yeast cells in phosphate-limited chemostat for dilution rates below and above approximately 0.2 h(-1) has been discussed in terms of the batch growth kinetic data and the change in the metabolic activity of the yeast cells. Immunological detection of a C-terminally myc epitope-tagged Pho84 fusion protein indicated derepressive expression of the Pho84 high-affinity P ( i ) transporter in the entire range of dilution rates employed in this study. Phosphate transport activity mediated by Pho84 transporter was highest at very low dilution rates, i.e. 0.08-0.1 h(-1), corresponding to conditions in which the amount of synthesized Pho84 was at its maximum.
Avdelning/ar
Publiceringsår
2007
Språk
Engelska
Sidor
17-25
Publikation/Tidskrift/Serie
Journal of Industrial Microbiology & Biotechnology
Volym
34
Issue
1
Dokumenttyp
Artikel i tidskrift
Förlag
Springer
Ämne
- Production Engineering, Human Work Science and Ergonomics
Nyckelord
- phosphate
- Pho84 transporter
- growth kinetics
- saccharomyces cerevisiae
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1367-5435