Cereal Byproducts Have Prebiotic Potential in Mice Fed a High-Fat Diet
Författare
Summary, in English
Barley husks, rye bran, and a fiber residue from oat milk production were processed by heat pretreatment, various
separation steps, and treatment with an endoxylanase in order to improve the prebiotic potential of these cereal byproducts.
Metabolic functions were intended to improve along with improved microbial activity. The products obtained were included in a
high-fat mouse diet so that all diets contained 5% dietary fiber. In addition, high-fat and low-fat controls as well as partially
hydrolyzed guar gum were included in the study. The soluble fiber product obtained from rye bran caused a significant increase
in the bifidobacteria (log copies of 16S rRNA genes; median (25−75 percentile): 6.38 (6.04−6.66) and 7.47 (7.30−7.74),
respectively; p < 0.001) in parallel with a tendency of increased production of propionic acid and indications of improved
metabolic function compared with high-fat fed control mice. The oat-derived product caused an increase in the pool of cecal
propionic (from 0.62 ± 0.12 to 0.94 ± 0.08) and butyric acid (from 0.38 ± 0.04 to 0.60 ± 0.04) compared with the high-fat
control, and it caused a significant increase in lactobacilli (log copies of 16S rRNA genes; median (25−75 percentile): 6.83
(6.65−7.53) and 8.04 (7.86−8.33), respectively; p < 0.01) in the cecal mucosa. However, no changes in measured metabolic
parameters were observed by either oat or barley products.
separation steps, and treatment with an endoxylanase in order to improve the prebiotic potential of these cereal byproducts.
Metabolic functions were intended to improve along with improved microbial activity. The products obtained were included in a
high-fat mouse diet so that all diets contained 5% dietary fiber. In addition, high-fat and low-fat controls as well as partially
hydrolyzed guar gum were included in the study. The soluble fiber product obtained from rye bran caused a significant increase
in the bifidobacteria (log copies of 16S rRNA genes; median (25−75 percentile): 6.38 (6.04−6.66) and 7.47 (7.30−7.74),
respectively; p < 0.001) in parallel with a tendency of increased production of propionic acid and indications of improved
metabolic function compared with high-fat fed control mice. The oat-derived product caused an increase in the pool of cecal
propionic (from 0.62 ± 0.12 to 0.94 ± 0.08) and butyric acid (from 0.38 ± 0.04 to 0.60 ± 0.04) compared with the high-fat
control, and it caused a significant increase in lactobacilli (log copies of 16S rRNA genes; median (25−75 percentile): 6.83
(6.65−7.53) and 8.04 (7.86−8.33), respectively; p < 0.01) in the cecal mucosa. However, no changes in measured metabolic
parameters were observed by either oat or barley products.
Avdelning/ar
- Molecular Nutrition
- Bioteknik
- Avdelningen för livsmedel och läkemedel
- Molekylär endokrinologi
- Biokemi och Strukturbiologi
- Food for Health Science Centre, Kemicentrum
- Food for Health Science Centre
- EXODIAB: Excellence of Diabetes Research in Sweden
Publiceringsår
2014-08
Språk
Engelska
Sidor
8169-8178
Publikation/Tidskrift/Serie
Journal of Agricultural and Food Chemistry
Volym
62
Issue
32
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
The American Chemical Society (ACS)
Ämne
- Agricultural Science, Forestry and Fisheries
Nyckelord
- dietary fiber
- C57BL/6 mice
- gut microbiota
- short-chain fatty acids (SCFAs)
- xylanase
Status
Published
Projekt
- ANTIDIABETIC FOOD CENTRE
Forskningsgrupp
- Molecular Nutrition
- Molecular Endocrinology
ISBN/ISSN/Övrigt
- ISSN: 0021-8561