Inflammatory Markers in Huntington’s Disease Plasma – A Robust NanoLC-MRM-MS Assay Development

Summary, in English

The development of a quantitative multiplex reaction monitoring (MRM) assay is presented for the measurements of seven inflammatory markers in human plasma, using nanoLC-MS/MS analysis with stable isotope dilution strategy. We report a robust and sensitive, mass spectrometry based, quantitative assay with a relative standard deviation of <15% that accounts for the entire sample processing, while the analytical reproducibility of the assay was 3.6%. We used plasma from Huntington´s disease (HD) gene carriers and healthy controls to compare our MRM method with established antibody based methods. Interestingly, we found a good agreement between assays for the measurement of C-reactive protein (CRP).

• Författare: Melinda Rezeli; Ákos Végvári; Edina Silajdzic; Maria Björkqvist; Sarah Tabrizi; Thomas Laurell; György Marko-Varga
• Avdelning/ar: Avdelningen för Biomedicinsk teknik; Biomarkörer vid hjärnsjukdomar; MultiPark: Multidisciplinary research focused on Parkinson´s disease
• Publiceringsår: 2014
• Språk: Engelska
• Sidor: 68-75
• Publikation/Tidskrift/Serie: EuPA Open Proteomics
• Volym: 3
• Länkar: http://dx.doi.org/10.1016/j.euprot.2014.02.003
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Medical Engineering
• Nyckelord: multiple reaction monitoring; complement components; C-reactive protein; Huntington´s disease
• Status: Published
• Forskningsgrupp: Biomarkers in Brain Disease
• ISBN/ISSN/Övrigt: ISSN: 2212-9685