Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Proteome-wide selected reaction monitoring assays for the human pathogen Streptococcus pyogenes.

In English

Författare

Summary, in English

Selected reaction monitoring mass spectrometry (SRM-MS) is a targeted proteomics technology used to identify and quantify proteins with high sensitivity, specificity and high reproducibility. Execution of SRM-MS relies on protein-specific SRM assays, a set of experimental parameters that requires considerable effort to develop. Here we present a proteome-wide SRM assay repository for the gram-positive human pathogen group A Streptococcus. Using a multi-layered approach we generated SRM assays for 10,412 distinct group A Streptococcus peptides followed by extensive testing of the selected reaction monitoring assays in >200 different group A Streptococcus protein pools. Based on the number of SRM assay observations we created a rule-based selected reaction monitoring assay-scoring model to select the most suitable assays per protein for a given cellular compartment and bacterial state. The resource described here represents an important tool for deciphering the group A Streptococcus proteome using selected reaction monitoring and we anticipate that concepts described here can be extended to other pathogens.

Publiceringsår

2012

Språk

Engelska

Publikation/Tidskrift/Serie

Nature Communications

Volym

3

Dokumenttyp

Artikel i tidskrift

Förlag

Nature Publishing Group

Ämne

  • Infectious Medicine

Status

Published

Forskningsgrupp

  • epIgG
  • Infection Medicine Proteomics
  • SEBRA Sepsis and Bacterial Resistance Alliance

ISBN/ISSN/Övrigt

  • ISSN: 2041-1723