Molecular microheterogeneity of prostate specific antigen in seminal fluid by mass spectrometry.
Författare
Summary, in English
OBJECTIVES: Prostate specific antigen (PSA) is a widely used and clinically valuable marker for prostate disease. In order to enable the development of new PSA assays and progress the understanding of the biology of PSA we have analyzed PSA in seminal plasma. Design and methods PSA in seminal plasma from men attending a fertility clinic and healthy controls was analyzed using SDS-PAGE, Western blotting and mass spectrometry. RESULTS: Using mass spectrometry, different forms of PSA could be identified in 1-9 bands seen on SDS-PAGE analysis of the respective sample. However, a majority of these molecular forms of PSA were not observed on Western blots. Enzymatic activity of PSA isoforms was demonstrated by sequencing data in zymogram gels. Multivariate analysis of clinical data revealed well-separated patient groups. CONCLUSIONS: We demonstrated that PSA in seminal plasma occurs in several isoforms, yet not all were detectable using an antibody based clinical routine method. The heterogeneity of PSA expression might be of clinical significance, by an improved patient phenotyping.
Avdelning/ar
- Avdelningen för Biomedicinsk teknik
- Bröstcancer-genetik
- Klinisk kemi, Malmö
- EpiHealth: Epidemiology for Health
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
Publiceringsår
2012
Språk
Engelska
Sidor
331-338
Publikation/Tidskrift/Serie
Clinical Biochemistry
Volym
45
Issue
4-5
Fulltext
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Biochemistry and Molecular Biology
Nyckelord
- ESI-LTQ FT-ICR
- MALDI LTQ Orbitrap XL
- infertility
- seminal plasma
- prostate specific antigen
- isoform
Status
Published
Forskningsgrupp
- Clinical Chemistry, Malmö
ISBN/ISSN/Övrigt
- ISSN: 1873-2933