Assignment of gene for coagulation factor V to chromosome 1 in man and to chromosome 13 in rat

Summary, in English

Two different factor V cDNA fragments were used as hydridization probes in the chromosomal assignment of the human and rat factor V genes. A 1.6-kb EcoRI fragment was used as a hybridization probe to analyze a panel of human-rodent somatic cell hybrids. Cosegregation of factor V specific DNA restriction fragments with human chromosome 1 was observed. In addition, a panel of rat-mouse somatic cell hybrids was analyzed with another human factor V cDNA probe to localize the gene for rat coagulation factor V. In the rat, the gene for coagulation factor V was found to be located in chromosome 13. This is the first gene in the rat to be localized to chromosome 13.

• Författare: Björn Dahlbäck; C. Hansson; M. Q. Islam; J. Szpirer; C. Szpirer; Åke Lundwall; G. Levan
• Avdelning/ar: Klinisk kemi, Malmö
• Publiceringsår: 1988
• Språk: Engelska
• Sidor: 14-509
• Publikation/Tidskrift/Serie: Somatic Cell and Molecular Genetics
• Volym: 14
• Issue: 5
• Länkar: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=3175767
• Dokumenttyp: Artikel i tidskrift
• Förlag: Springer
• Ämne: Medicinal Chemistry
• Nyckelord: DNA Probes; Molecular; Cloning; Pair 1; Human; *Chromosomes; *Chromosome Mapping; Animals; Southern; Blotting; Factor V/*genetics; Humans; Hybrid Cells; Nucleic Acid Hybridization; Rats; Research Support; Non-U.S. Gov't
• Status: Published
• Forskningsgrupp: Clinical Chemistry, Malmö
• ISBN/ISSN/Övrigt: ISSN: 0740-7750