Characterization of water-soluble hemicelluloses from spruce and aspen employing SEC/MALDI mass spectroscopy
Författare
Summary, in English
Partly depolymerized hemicelluloses isolated from wood chips of spruce and aspen employing microwave treatment were resolved using size-exclusion chromatography (SEC) into oligo- and polysaccharide fractions containing components with a narrow range of sizes, as determined by MALDI mass spectroscopy. The degree of substitution with acetyl moieties (DS) was also calculated on the basis of the MALDI-MS spectra obtained prior to and following deacetylation. For spruce hemicelluloses, the low molecular mass fraction contained small arabino-4-O-methylglucuronoxylan oligosaccharides, with DP values ranging from 4 to ~20, separated primarily on the basis of their charge density. The fraction eluted last consisted of an O-acetyl-(galacto)glucomannan polysaccharide of peak-average DP value (DPp) 14. The degree of substitution with acetyl groups (DS) decreased with decreasing DP, a value DS of 0.39 being obtained for the fraction with DPp 12. For the aspen hemicelluloses, the SEC fractions eluted first contained an acidic O-acetyl-4-O-methylglucuronoxylan polysaccharide with DP ranging from 10 to ~28 and an average DS of ~0.75. The fractions eluted last consisted of oligosaccharide mixtures composed primarily of small neutral O-acetyl-xylooligosaccharides (DPp 6, DS 0.41), together with minor quantities of an O-acetyl-glucomannan.
Avdelning/ar
Publiceringsår
2002
Språk
Engelska
Sidor
711-717
Publikation/Tidskrift/Serie
Carbohydrate Research
Volym
337
Issue
8
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Biological Sciences
Nyckelord
- Aspen wood
- Spruce wood
- Hemicellulose
- O-Acetylation
- Mannan
- Xylan
- Size-exclusion Chromatography
- Microwave treatment
- MALDI-TOF-MS
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1873-426X