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Derivation of a xeno-free human ES cell line.

Författare

  • Catharina Ellerström
  • Raimund Strehl
  • Karina Moya
  • Katarina Andersson
  • Christina Bergh
  • Kersti Lundin
  • Johan Hyllner
  • Henrik Semb

Summary, in English

Elimination of all animal material during both the derivation and long-term culture of human embryonic stem cells (hESCs) is necessary prior to future application of hESCs in clinical cell therapy. The potential consequences of transplanting xeno-contaminated hESCs into patients, such as an increased risk of graft rejection [STEM CELLS 2006;24:221229] and the potential transfer of nonhuman pathogens, make existing hESC lines unsuitable for clinical applications. To avoid xeno-contamination during derivation and culture of hESCs, we first developed a xeno-free medium supplemented with human serum, which supports long-term (> 50 passages) culture of hESCs in an undifferentiated state. To enable derivation of new xeno-free hESCs, we also established xeno-free human foreskin fibroblast feeders and replaced immunosurgery, which involves the use of guinea pig complement, with a modified animal-product-free derivation procedure. Here, we report the establishment and characterization (> 20 passages) of a xeno-free pluripotent diploid normal hESC line, SA611.

Publiceringsår

2006

Språk

Engelska

Sidor

2170-2176

Publikation/Tidskrift/Serie

Stem Cells

Volym

24

Issue

10

Dokumenttyp

Artikel i tidskrift

Förlag

AlphaMed Press

Ämne

  • Cell and Molecular Biology

Nyckelord

  • human feeders
  • human serum
  • clinical
  • therapies
  • human embryonic stem cell

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1549-4918