Mouse ten-m/Odz is a new family of dimeric type II transmembrane proteins expressed in many tissues
Författare
Summary, in English
The Drosophila gene ten-m/odz is the only pair rule gene identified to date which is not a transcription factor. In an attempt to analyze the structure and the function of ten-m/odz in mouse, we isolated four murine ten-m cDNAs which code for proteins of 2,700-2, 800 amino acids. All four proteins (Ten-m1-4) lack signal peptides at the NH2 terminus, but contain a short hydrophobic domain characteristic of transmembrane proteins, 300-400 amino acids after the NH2 terminus. About 200 amino acids COOH-terminal to this hydrophobic region are eight consecutive EGF-like domains. Cell transfection, biochemical, and electronmicroscopic studies suggest that Ten-m1 is a dimeric type II transmembrane protein. Expression of fusion proteins composed of the NH2-terminal and hydrophobic domain of ten-m1 attached to the alkaline phosphatase reporter gene resulted in membrane-associated staining of the alkaline phosphatase. Electronmicroscopic and electrophoretic analysis of a secreted form of the extracellular domain of Ten-m1 showed that Ten-m1 is a disulfide-linked dimer and that the dimerization is mediated by EGF-like modules 2 and 5 which contain an odd number of cysteines. Northern blot and immunohistochemical analyses revealed widespread expression of mouse ten-m genes, with most prominent expression in brain. All four ten-m genes can be expressed in variously spliced mRNA isoforms. The extracellular domain of Ten-m1 fused to an alkaline phosphatase reporter bound to specific regions in many tissues which were partially overlapping with the Ten-m1 immunostaining. Far Western assays and electronmicroscopy demonstrated that Ten-m1 can bind to itself.
Publiceringsår
1999
Språk
Engelska
Sidor
563-577
Publikation/Tidskrift/Serie
Journal of Cell Biology
Volym
145
Issue
3
Dokumenttyp
Artikel i tidskrift
Förlag
Rockefeller University Press
Ämne
- Cancer and Oncology
- Ophthalmology
- Infectious Medicine
- Cell and Molecular Biology
Nyckelord
- epidermal growth factor
- pair rule
- ten-m/odz
- transmembrane protein
Status
Published
Forskningsgrupp
- Vessel Wall Biology
ISBN/ISSN/Övrigt
- ISSN: 0021-9525