Quantification of Membrane Proteins Using Nonspecific Protease Digestions
Författare
Summary, in English
We present a mass spectrometry-based method for the identification and quantification of membrane proteins using the low-specificity protease Proteinase K, at very high pH, to digest proteins isolated by a modified SDS-PAGE protocol. The resulting peptides are modified with a fragmentation-directing isotope labeled tag. We apply the method to quantify differences in membrane protein expression of Bacillus subtilis grown in the presence or absence of glucose.
Publiceringsår
2009
Språk
Engelska
Sidor
5666-5673
Publikation/Tidskrift/Serie
Journal of Proteome Research
Volym
8
Issue
12
Dokumenttyp
Artikel i tidskrift
Förlag
The American Chemical Society (ACS)
Ämne
- Immunology in the medical area
Nyckelord
- membrane proteins
- N-terminal labeling
- quantification
- relative
- proteinase K
- Bacillus subtilis
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1535-3893