Capturing ion exchanger-bound infectious pancreatic necrosis virus - design and application for large volume water samples
Författare
Summary, in English
A method was designed for rapid and reliable demonstration of the presence of infectious pancreatic necrosis virus retrieved from 5 1 water samples. Viruses together with an added carrier protein were adsorbed to a resin of an added anion exchanger. Then the resin was collected rapidly and quantitatively through a specific device which we designed. The resin was transferred to a column from which the viruses were eluted, and subsequently further concentrated by acid precipitation, followed by SDS-polyacrylamide eel electrophoresis and Western blot analysis. Serological results were obtained within 24 h after the water sample was introduced into the laboratory. Proteins were recovered at an efficiency between 70 and 80% and the total concentration factor ranged between 150000 and 250000 times, depending on the requirements and the methods of choice.
Avdelning/ar
Publiceringsår
2003
Språk
Engelska
Sidor
173-178
Publikation/Tidskrift/Serie
Journal of Virological Methods
Volym
110
Issue
2
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Biological Sciences
Nyckelord
- ion exchanger
- IPNV
- virus concentration
- filtration device
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1879-0984