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Assignment of the human gene for beta-microseminoprotein (MSMB) to chromosome 10 and demonstration of related genes in other vertebrates

Författare

Summary, in English

The gene for beta-microseminoprotein MSMB has been studied by DNA hybridization and molecular cloning techniques. Comparative analysis of restriction endonuclease digests of the cloned gene and of leukocyte DNA strongly suggested that the gene is present in a single copy in the haploid human genome. By Southern blot analysis of DNA from somatic cell hybrids, the gene was assigned to chromosome 10. The coding nucleotides of the human gene are separated into four exons by relatively large introns. A related gene might be present in other mammals, birds, and amphibians as revealed by DNA hybridization under conditions of low stringency.

Publiceringsår

1991

Språk

Engelska

Sidor

4-920

Publikation/Tidskrift/Serie

Genomics

Volym

11

Issue

4

Dokumenttyp

Artikel i tidskrift

Förlag

Academic Press

Ämne

  • Medicinal Chemistry

Nyckelord

  • *Prostatic Secretory Proteins
  • Molecular Sequence Data
  • Humans
  • Dna
  • Molecular
  • Cloning
  • Pair 10
  • Human
  • *Chromosomes
  • Chromosome Mapping
  • Southern
  • Blotting
  • Animals
  • Base Sequence
  • Proteins/*genetics
  • Research Support
  • Non-U.S. Gov't
  • Restriction Mapping
  • Semen/*metabolism
  • Seminal Plasma Proteins
  • Vertebrates/genetics

Status

Published

Forskningsgrupp

  • Clinical Chemistry, Malmö

ISBN/ISSN/Övrigt

  • ISSN: 1089-8646