PIXE Analysis of Blood Serum Proteins, Separated by Gel Filtration
Författare
Summary, in English
Gel filtration of blood serum was carried out with Sephadex G-200 gel and tris buffer at pH 7.8. The amount of protein in each of about 100 collected fractions was monitored using UV absorption. The fractions were concentrated and deposited onto Kimfol backing foils.
From the PIXE results distributions of S, Fe, Cu and Zn were obtained in relation to the protein peaks, while elements like K, Ca and Br appeared at the position corresponding to small molecular sizes. Quantification, taking into account the intermediate thicknesses of the samples, gave reasonable values.
By adjusting the pH of the tris buffer with acetate instead of HCl, an increase in sensitivity was achieved, as the Cl is an important source of electron bremsstrahlung. By low temperature ashing still better detection limits can be reached, but at the expense of the loss of volatile elements.
From the PIXE results distributions of S, Fe, Cu and Zn were obtained in relation to the protein peaks, while elements like K, Ca and Br appeared at the position corresponding to small molecular sizes. Quantification, taking into account the intermediate thicknesses of the samples, gave reasonable values.
By adjusting the pH of the tris buffer with acetate instead of HCl, an increase in sensitivity was achieved, as the Cl is an important source of electron bremsstrahlung. By low temperature ashing still better detection limits can be reached, but at the expense of the loss of volatile elements.
Avdelning/ar
Publiceringsår
1984
Språk
Engelska
Sidor
373-376
Publikation/Tidskrift/Serie
Nuclear Instruments and Methods in Physics Research B
Volym
3
Issue
1-3
Dokumenttyp
Artikel i tidskrift
Förlag
North-Holland
Ämne
- Subatomic Physics
- Production Engineering, Human Work Science and Ergonomics
Nyckelord
- serum proteins
- gel filtration
- PIXE
- elemental composition
Status
Published