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Permanent activation of HMGA2 in lipomas mimics its temporal physiological activation linked to the gain of adipose tissue

Författare

  • Helge W. Thies
  • Ingo Nolte
  • Heiner Wenk
  • Fredrik Mertens
  • Joern Bullerdiek
  • Dominique N. Markowski

Summary, in English

Objective: In this study the activation of HMGA2 and overexpression by FGF1-driven stimulation of adipose tissue derived stem cells (ADSCs) in adipose tissue tumors were analyzed. In addition, the expression of HMGA2 and PPAR-gamma mRNA were quantified in canine subcutaneous abdominal adipose tissue from normal and overweight purebred dogs. Design and Methods: ADSCs and adipose tissue explants stimulated with FGF1 followed by gene expression analyses of HMGA2 and p14Arf using Western-blot and qRT-PCR. Furthermore, canine subcutaneous white adipose tissue (WAT) were analyzed by qRT-PCR for their expression of HMGA2 and PPAR-gamma. Results: ADSCs and adipose tissue explants are able to execute a HMGA2 response upon FGF1 stimulation. FGF1 enhances proliferation of ADSCs by a HMGA2-dependent mechanism. In lipomas increase of HMGA2 is accompanied by increased expression of p14Arf. Furthermore, a significantly elevated level of HMGA2 in overweight dogs and a negative correlation between the expression of HMGA2 and PPAR-gamma in subcutaneous cWAT were noted. Conclusions: These results suggest that WAT contains cells that as essential part of adipogenesis up-regulate HMGA2 resulting from growth factor stimulation. In subgroups of lipoma, constitutive activation of HMGA2 due to rearrangements replaces the temporal response triggered by growth factors.

Avdelning/ar

Publiceringsår

2014

Språk

Engelska

Sidor

141-150

Publikation/Tidskrift/Serie

Obesity

Volym

22

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

Nature Publishing Group

Ämne

  • Nutrition and Dietetics

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1930-739X