Vital staining of fungi in pure cultures and in soil with fluorescein diacetate
Författare
Summary, in English
A method for vital staining of fungal mycelium with fluorescein diacetate (FDA) is described. In experiments with pure cultures, good correlation was obtained between relative staining efficiency, growth rate, and respiration. FDA thus appears to be a true vital stain, in that it stains only metabolically active mycelia.
In fresh soil suspensions stained with FDA, brightly fluorescent hyphae and portions of hyphae were observed. The applicability of the method for measurement of active mycelium in the soil is discussed.
In fresh soil suspensions stained with FDA, brightly fluorescent hyphae and portions of hyphae were observed. The applicability of the method for measurement of active mycelium in the soil is discussed.
Avdelning/ar
Publiceringsår
1977
Språk
Engelska
Sidor
59-63
Publikation/Tidskrift/Serie
Soil Biology & Biochemistry
Volym
9
Issue
1
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Biological Sciences
Status
Published
Forskningsgrupp
- Microbial Ecology
ISBN/ISSN/Övrigt
- ISSN: 0038-0717