Immunoglobulin production induced by CD57+ GC-derived helper T cells in vitro requires addition of exogenous IL-2

Summary, in English

Germinal centers (GC) are well-defined areas in lymphoid organs were B cells proliferate and differentiate in response to T-cell-dependent antigens. The GC comprises B cells, follicular dendritic cells, tangible body macrophages, and a low number of CD4⁺ T cells. A large portion of these T cells expresses CD57. We have examined the ability of the CD4⁺CD57⁺ GC T cells to become activated and to take part in B cell activation processes, These T cells coexpress CD45RO, CD69, CD28, and upon mitogenic stimulation CD25, The cell population was found neither to containe nor to be able to produce any specific mRNA for IL-2, IL-4, and IFN-γ upon activation. Levels of mRNA encoding CD40 ligand was also undetectable under similar conditions. Furthermore, in contrast to ordinary CD4⁺ T cells, this population expressing CD57 was unable to induce B cells to Ig production in the presence of pokeweed mitogen or SEA unless IL-2 was added to the cultures, However, despite their apparent lack of function CD4⁺CD57⁺ GC T cells were found to rescue GC B cells from cell death in vitro to the same extent as CD4⁺CD57^- T(h) cells, The phenotypical and functional differences found between these T cells and regular T(h)-cells suggest that they either represent a T cell subset with distinct properties within the GC yet to be determined or that they represent T cells, Irate in the immune response, having lost most of their original functions and capabilities.

• Författare: Eva Andersson; Katarina Dahlenborg; Mats Ohlin; Carl A K Borrebaeck; Roland Carlsson
• Avdelning/ar: Institutionen för immunteknologi
• Publiceringsår: 1996-05-01
• Språk: Engelska
• Sidor: 166-173
• Publikation/Tidskrift/Serie: Cellular Immunology
• Volym: 169
• Issue: 2
• Länkar: http://dx.doi.org/10.1006/cimm.1996.0107
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 0008-8749