Regulation of bone sialoprotein mRNA by steroid hormones.

Summary, in English

In this report we demonstrate an increase in the steady-state level of bone sialoprotein (BSP) mRNA in rat calvaria and a rat osteosarcoma cell line (ROS 17/2.8) after treatment with the synthetic glucocorticoid, dexamethasone. In contrast, 1.25-dihydroxyvitamin D3 reduced the amount of BSP mRNA in calvaria and inhibited the dexamethasone induction in ROS 17/2.8 cells. The increase in BSP mRNA is most likely due to an increase in the transcriptional rate. The stability of mRNA was unchanged after dexamethasone treatment with a half-life of approximately 5 h. Nuclear transcription experiments with nuclei isolated from ROS 17/2.8 cells showed an increased BSP mRNA synthesis in cells treated with dexamethasone.

• Författare: Åke Oldberg; B Jirskog-Hed; Sara Axelsson; Dick Heinegård
• Avdelning/ar: Åke Oldberg´s group; Öron-, näs- och halssjukdomar, Lund; Institutionen för kliniska vetenskaper, Lund
• Publiceringsår: 1989
• Språk: Engelska
• Sidor: 3183-3186
• Publikation/Tidskrift/Serie: Journal of Cell Biology
• Volym: 109
• Issue: 6 Pt 1
• Länkar: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115918/
• Dokumenttyp: Artikel i tidskrift
• Förlag: Rockefeller University Press
• Ämne: Otorhinolaryngology
• Nyckelord: Messenger: genetics; Messenger: drug effects; RNA; Genes: drug effects; Gene Expression Regulation: drug effects; Dexamethasone: pharmacology; Calcitriol: pharmacology; Bone and Bones: drug effects; Bone and Bones: metabolism; Sialoglycoproteins: genetics; Transcription; Genetic: drug effects
• Status: Published
• Forskningsgrupp: Åke Oldberg´s group
• ISBN/ISSN/Övrigt: ISSN: 0021-9525