A subset of dendritic cells express joining chain (J-chain) protein

Summary, in English

Joining chain (J-chain) is well known as an integrated component of dimeric immunoglobulin A (IgA) and pentameric IgM. We show here that the J-chain protein is also expressed in a subset of CD11c+ dendritic cells (DC) in C57BL/6 mice. J-chain knockout mice (J−/− mice) had a reduced fraction of CD4−/CD8α+ and mPDCA-1+ DC in the spleen. J−/− mice also had reduced levels of RNA for the immunoregulatory enzyme indoleamine 2,3-dioxygenase (IDO) in the spleen. Furthermore, in lymph nodes from C57BL/6 mice the majority of J-chain-expressing CD11c+ cells also expressed IDO, while the number of IDO-expressing cells in lymph nodes and the amount of IDO protein in splenic CD11c+ cells were reduced in J−/− mice. Also, J−/− mice had a lower ratio of kynurenine/tryptophan in serum compared to C57BL/6 mice, indicating a lower overall IDO activity in J−/− mice. We also show that J−/− mice are less susceptible to tolerance induction than C57BL/6 mice. In conclusion, our data show that J-chain protein is expressed outside the B-cell compartment in a subset of immunoregulatory DC that are compromised in animals that cannot express J-chain.

• Författare: Eva Källberg; Tomas Leanderson
• Avdelning/ar: Immunology
• Publiceringsår: 2008
• Språk: Engelska
• Sidor: 590-599
• Publikation/Tidskrift/Serie: Immunology
• Volym: 129
• Issue: 4
• Länkar: http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=18028376&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum; http://dx.doi.org/10.1111/j.1365-2567.2007.02733.x
• Dokumenttyp: Artikel i tidskrift
• Förlag: Wiley-Blackwell
• Ämne: Immunology in the medical area
• Status: Published
• Forskningsgrupp: Immunology
• ISBN/ISSN/Övrigt: ISSN: 0019-2805