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Macromolecular geometries determined with field-flow fractionation and their impact on the overlap concentration.

In English

Författare

Summary, in English

In this paper we aim to understand the size/conformation relationship in waxy barley starch, a polydisperse and ultrahigh molar mass biomacromolecule. Characterizations are performed with asymmetrical flow field-flow fractionation (AsFlFFF). Furthermore, we study the effect of homogenization on the molar mass, rms radius (r rms) and hydrodynamic radius (r h). For the untreated sample, the macromolecules are elongated objects with low apparent density. As a result of homogenization, molar mass, and r rms decrease, while r h remains unaffected. The process also induces an increase, and scaling with size, of apparent density as well as changes in conformation, represented qualitatively by r rms/ r h. Finally, results from AsFlFFF are compared with viscosimetry and discussed in terms of concentration and close-packing in relation to macromolecular shape and conformation. Hence, the results show that AsFlFFF and our novel methodology enable the determination of several physical properties with high relevance for the solution behavior of polydisperse macromolecules.

Avdelning/ar

Publiceringsår

2008

Språk

Engelska

Sidor

1684-1690

Publikation/Tidskrift/Serie

Biomacromolecules

Volym

9

Issue

6

Dokumenttyp

Artikel i tidskrift

Förlag

The American Chemical Society (ACS)

Ämne

  • Analytical Chemistry
  • Food Engineering

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1526-4602