A new method for isolation of purified genomic DNA from haemosporidian parasites inhabiting nucleated red blood cells
Författare
Summary, in English
During the last 10 years, whole genomes have been sequenced from an increasing number of organisms. However, there is still no data on complete genomes of avian and lizard Plasmodium spp. or other haemosporidian parasites. In contrast to mammals, bird and reptile red blood cells have nuclei and thus blood of these vertebrates contains high amount of host DNA; that complicates preparation of purified template DNA from haemosporidian parasites, which has been the main obstacle for genomic studies of these parasites. In the present study we describe a method that generates large amount of purified avian haemosporidian DNA. The method is based on a unique biological feature of haemosporidian parasites, namely that mature gametocytes in blood can be induced to exflagellate in vitro. This results in the development of numerous microgametes, which can be separated from host blood cells by simple centrifugation. Our results reveal that this straight forward method provides opportunities to collect pure parasite DNA material, which can be used as a template for various genetic analyses including whole genome sequencing of haemosporidians infecting birds and lizards. (c) 2012 Elsevier Inc. All rights reserved.
Avdelning/ar
Publiceringsår
2013
Språk
Engelska
Sidor
275-280
Publikation/Tidskrift/Serie
Experimental Parasitology
Volym
133
Issue
3
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Biological Sciences
Nyckelord
- Avian malaria
- Haemosporidians
- Haemoproteus tartakovskyi
- hSISKIN1
- Template
- DNA purification
- Next generation sequencing
Status
Published
Projekt
- Malaria in birds
Forskningsgrupp
- Molecular Ecology and Evolution Lab
ISBN/ISSN/Övrigt
- ISSN: 0014-4894