Mechanisms of adaptation to the effects of ethanol on activation of phospholipase C in NG 108-15 cells

Summary, in English

In this study the effect of different times of exposure to ethanol (1-7 days, 100 mM) on bradykinin and GTP(S)-stimulated activation of phospholipase C in NG 108-15 cells and on the binding of [3H]bradykinin to its receptors was investigated. Ethanol attenuated both agonist and GTP-analogue-induced hydrolysis of phosphoinositides for a period of up to 4 days of treatment, while exerting no effect on binding to bradykinin receptors. However, after 7 days of exposure to ethanol, the agonist-induced activation of phospholipase C was completely resistant to the inhibitory effects of alcohol. This finding correlated to a change in the affinity of the bradykinin receptor population after 7 days of treatment. The results indicate that bradykinin-induced breakdown of phosphatidylinositol 4,5-bisphosphate adapts to the effects of ethanol, after long-term treatment. Possible adaptative changes taking place at the level of the G protein(s), may induce a shift in the affinity of the receptor population and, consequently, serve as a compensatory mechanism to counteract the inhibitory effect of ethanol.

• Författare: F D Rodriguez; Per Simonsson; L Gustavsson; Christer Alling
• Avdelning/ar: Klinisk kemi, Malmö; Avdelningen för klinisk kemi och farmakologi
• Publiceringsår: 1992
• Språk: Engelska
• Sidor: 1157-1164
• Publikation/Tidskrift/Serie: Neuropharmacology
• Volym: 31
• Issue: 11
• Länkar: http://dx.doi.org/10.1016/0028-3908(92)90012-E
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Pharmacology and Toxicology
• Nyckelord: NG 108-15 cell line; adaptation; inositol phosphates; bradykinin; G proteins; ethanol
• Status: Published
• Forskningsgrupp: Clinical Chemistry, Malmö
• ISBN/ISSN/Övrigt: ISSN: 1873-7064