HASH-1 and E2-2 are expressed in human neuroblastoma cells and form a functional complex

Summary, in English

The basic helix-loop-helix (bHLH) transcription factor mammalian achaete-scute homolog-1 (MASH-1 in mouse and HASH-1 in human) is essential for proper development of olfactory and most peripheral autonomic neurons, and for the formation of distinct neuronal circuits within the central nervous system. We have previously shown that HASH-1 is expressed in neuroblastoma tumors and cell lines, and in this study we have used the yeast two-hybrid system to isolate HASH-1 interacting proteins from a human neuroblastoma cDNA library. Two of the isolated clones contained cDNA from the E2-2 gene (also known as ITF2/SEF2-1). We show that E2-2 interacts with HASH-1 in both yeast and mammalian cells. The HASH-1/E2-2 complex binds an E-box (CACCTG) in vitro, and transactivates an E-box containing reporter construct in vivo. Furthermore, E2-2 seems to be one of the major HASH-1 interacting proteins in extracts from neuroblastoma cells. In conclusion, E2-2 forms a functional complex with HASH-1, and might therefore be involved in the development of specific parts of the central and peripheral nervous systems.

• Författare: Paula Persson; Annika Jögi; Anna Grynfeld; Sven Påhlman; Håkan Axelson
• Avdelning/ar: Institutionen för translationell medicin; Avdelningen för molekylärmedicin och genterapi
• Publiceringsår: 2000
• Språk: Engelska
• Sidor: 22-31
• Publikation/Tidskrift/Serie: Biochemical and Biophysical Research Communications
• Volym: 274
• Issue: 1
• Länkar: http://dx.doi.org/10.1006/bbrc.2000.3090
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Biological Sciences
• Nyckelord: SEF2-1; neuroblastoma; HASH-1; sympathetic nervous system; E2-2; bHLH; differentiation; yeast two-hybrid
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1090-2104