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Mass spectrometric monitoring of in vitro formed ad ducts between hexahydrophthalic anhydride (HHPA) and cysteine and lysine

In English

Författare

Summary, in English

The in vitro formed adducts between hexahydrophthalic anhydride (HHPA) and cysteine and lysine were monitored by mass spectrometry. Freshly synthesized N-acetyl-S-hexahydrophthaloyl-L-cysteine was dissolved in dry acetonitrile and added to a solution of N-acetyl-L-lysine in 0.1 M sodium phosphate buffer (pH 7.4) an incubated at 37°C. The reactions were monitored by LC/MS on a triple quadrupole mass spectrometer with electrospray ionization coupled to a HPLC system from Perkin Elmer. The results show that there was a rapid rearrangement of HHPA from most of the cysteines to the lysines, and that HHPA-adducts of cysteine will be transferred to lysine when encountered.

Publiceringsår

2002

Språk

Engelska

Sidor

325-326

Publikation/Tidskrift/Serie

Proceedings 50th ASMS Conference on Mass Spectrmetry and Allied Topics

Dokumenttyp

Konferensbidrag

Förlag

American Society for Mass Spectrometry, Santa Fe, NM, 87505, United States

Ämne

  • Environmental Health and Occupational Health

Nyckelord

  • Electrospray ionization (ESI)
  • Multiple reaction monitoring (MRM)
  • Hexahydrophthalic anhydride (HHPA)
  • Ion spray voltage

Conference name

Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Conference date

2002-06-02 - 2002-06-06

Conference place

Orlando, FL, United States

Status

Published