Active zone protein expression changes at the key stages of cerebellar cortex neurogenesis in the rat
Författare
Summary, in English
Signal transduction and neurotransmitter release in the vertebrate central nervous system are confined to the structurally complex presynaptic electron dense projections called "active zones." Although the nature of these projections remains a mystery, genetic and biochemical work has provided evidence for the active zone (AZ) associated proteins i.e. Piccolo/Aczonin, Bassoon, RIM1/Unc10, Munc13/Unc13, Liprin-alpha/SYD2/Dliprin and ELKS/CAST/BRP and their specific molecular functions. It still remains unclear, however, what their precise contribution is to the AZ assembly. In our project, we studied in Wistar rats the temporal and spatial distribution of AZ proteins and their colocalization with Synaptophysin in the developing cerebellar cortex at key stages of cerebellum neurogenesis. Our study demonstrated that AZ proteins were already present at the very early stages of cerebellar neurogenesis and exhibited distinct spatial and temporal variations in immunoexpression throughout the course of the study. Colocalization analysis revealed that the colocalization pattern was time-dependent and different for each studied protein. The highest collective mean percentage of colocalization (>85%) was observed at postnatal day (PD) 5, followed by PD10 (>83%) and PD15 (>80%). The findings of our study shed light on AZ protein immunoexpression changes during cerebellar cortex neurogenesis and help frame a hypothetical model of AZ assembly. (C) 2013 Elsevier GmbH. All rights reserved.
Avdelning/ar
Publiceringsår
2013
Språk
Engelska
Sidor
616-625
Publikation/Tidskrift/Serie
Acta Histochemica
Volym
115
Issue
6
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Biochemistry and Molecular Biology
Nyckelord
- Active zone
- Active zone proteins
- Rat
- Cerebellar cortex
- Neurogenesis
- Synaptogenesis
- Immunofluorescence
Status
Published
Forskningsgrupp
- Neural Plasticity and Repair
ISBN/ISSN/Övrigt
- ISSN: 0065-1281