Temperature-dependent formation and photorepair of DNA damage induced by UV-B radiation in suspension-cultured tobacco cells
Författare
Summary, in English
Two photoproducts of DNA damage, i.e. cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts (6-4PPs), induced by UV-B radiation in suspension-cultured tobacco cells were quantified by enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies. CPDs and 6-4PPs were induced in tobacco cells by UV-B radiation. Photorepair of CPDs was faster than that of 6-4PPs. UV-B radiation induces formation of CPDs and 6-4PPs even at 0 °C, but low temperature significantly decreases the UV-B-induced (in contrast to UV-C-induced) formation of CPDs and 6-4PPs. Low temperature also retarded the removal of CPDs and 6-4PPs under white light, and almost no photorepair of CPDs and 6-4PPs was detected at 0 °C. When purified DNA from tobacco cells grown in darkness was irradiated with UV-B, formation of CPDs and 6-4PPs took place at the same speed at different temperatures. It indicated that formation of CPDs and 6-4PPs induced by UV-B was temperature-independent in a non-cellular system. Based on our results for suspension-cultured tobacco cells, not only the photorepair but also UV-B-induced formation of CPDs and 6-4PPs are temperature-dependent.
This is the final, accepted and revised manuscript of this article. Use alternative location to go to the published article. Requires subscription.
This is the final, accepted and revised manuscript of this article. Use alternative location to go to the published article. Requires subscription.
Avdelning/ar
Publiceringsår
2002
Språk
Engelska
Sidor
67-72
Publikation/Tidskrift/Serie
Journal of Photochemistry and Photobiology, B: Biology
Volym
66
Issue
1
Fulltext
- Available as PDF - 373 kB
- Download statistics
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Biological Sciences
Status
Published
Projekt
- Photobiology
Forskningsgrupp
- Photobiology
ISBN/ISSN/Övrigt
- ISSN: 1011-1344