Serum cystatin C, determined by a rapid, automated particle-enhanced turbidimetric method, is a better marker than serum creatinine for glomerular filtration rate

Summary, in English

We describe a fully automated particle-enhanced turbidimetric assay for cystatin C in undiluted serum and EDTA-plasma. The throughput is 90 samples per hour and urgent samples can be analyzed in 7 min. The assay range (0.4-14.1 mg/L) covers the concentration range in health and disease. The within- and between-run imprecision is 0.9% and 2.2%, respectively. Analytical recovery of additions of recombinant cystatin C averaged 98%. Rheumatoid factors (< or = 323,000 IU/L), bilirubin (< or = 150 mumol/L), hemoglobin (< or = 1.2 g/L), and triglycerides (< or = 8.5 mmol/L) do not interfere in the assay. In view of the superior (by ROC analysis) diagnostic accuracy of serum concentrations of cystatin C for reduced glomerular filtration rate (GFR) in comparison with creatinine, cystatin C seems an attractive alternative to creatinine for estimation of GFR.

• Författare: Jan Kyhse-Andersen; Camilla Schmidt; Gunnar Nordin; Britta Andersson; Peter Nilsson-Ehle; Veronica Lindström; Anders Grubb
• Avdelning/ar: Avdelningen för klinisk kemi och farmakologi
• Publiceringsår: 1994
• Språk: Engelska
• Sidor: 1921-1926
• Publikation/Tidskrift/Serie: Clinical Chemistry
• Volym: 40
• Issue: 10
• Länkar: http://www.clinchem.org/cgi/reprint/40/10/1921
• Dokumenttyp: Artikel i tidskrift
• Förlag: American Association for Clinical Chemistry
• Ämne: Biochemistry and Molecular Biology
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 0009-9147