Localization of Sunitinib in in vivo Animal and in vitro Experimental Models by MALDI Mass Spectrometry Imaging
Författare
Summary, in English
The spatial distribution of an anti-cancer drug and its intended target within a tumor plays a major role on determining how effective the drug can be at tackling the tumor. This study provides data regarding the lateral distribution of sunitinib, an oral antiangiogenic receptor tyrosine kinase inhibitor using an in vitro animal model as well as an in vitro experimental model that involved deposition of a solution of sunitinib onto tissue sections. All tumor sections were analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging and compared with subsequent histology staining. Six tumors at four different time points after commencement of in vivo sunitinib treatment were examined to observe the patterns of drug uptake. The levels of sunitinib present in in vivo treated tumor sections increased continuously until day seven but a decrease was observed at day 10. Furthermore, the in vitro experimental model was adjustable to produce a drug level similar to that obtained in the in vivo model experiments. The distribution of sunitinib in tissue sections treated in vitro appeared to agree with the histological structure of tumors suggesting that this approach may be useful for testing drug update.
Publiceringsår
2015
Språk
Engelska
Sidor
2245-2253
Publikation/Tidskrift/Serie
Analytical and Bioanalytical Chemistry
Volym
407
Issue
8
Fulltext
- Available as PDF - 12 MB
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Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Springer
Ämne
- Analytical Chemistry
Nyckelord
- Mass spectrometry
- MALDI
- MALDI-MSI
- In vivo models
- Drug
- Sunitinib
- Colorectal adenocarcinoma
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1618-2642