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A stable, radioactive substrate emulsion for assay of lipoprotein lipase

In English

Författare

Summary, in English

A method is described for the assay of lipoprotein lipase, using a stable, radioactive substrate emulsion. Fatty acid-labeled trioleoylglycerol was emulsified by homogenization in glycerol with lecithin as detergent. This anhydrous emulsion was stable for at least six weeks. Substrate solutions for enzyme assay were prepared by diluting the emulsion with buffer containing serum and albumin. The fatty acid produced on hydrolysis was isolated in a one-step liquid-liquid partition system. Incubations with extracts of acetone powders from adipose tissue displayed characteristics of lipoprotein lipase activity, i.e., serum dependence and inhibition by NaCl and protamine. The method is rapid (less than 1 hour), sensitive and reproducible, and suitable for routine use.

Publiceringsår

1976

Språk

Engelska

Sidor

536-541

Publikation/Tidskrift/Serie

Journal of Lipid Research

Volym

17

Issue

5

Dokumenttyp

Artikel i tidskrift

Förlag

American Society for Biochemistry and Molecular Biology

Ämne

  • Medicinal Chemistry
  • Pharmacology and Toxicology

Nyckelord

  • adipose lipoprotein lipase tri[3H]oleoylglycerol
  • triglyceride
  • postheparin serum

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1539-7262